Applications | JP Scientific Ltd.

Environmental Analysis

Trace extraction of environmental pollutants from soil and water by direct-immersion SPME-LC-MS.

Supporting Research

Embedding Mixed Sorbents in Binder: Solid-Phase Microextraction Coating with Wide Extraction Coverage and Its Application in Environmental Water Analysis On-site high-throughput targeted and untargeted determinations of emerging contaminants.
Identification of Omitted Pollutants in Environmental Water via In Situ Thin-Film Microextraction Compared SPE to Thin-Film Microextraction (TFME) for extraction of pollutants from water. The authors demonstrated that TFME was able to extract water pollutants that are unextractable by SPE alone.

Agricultural Residue Testing

Fast, low-solvent extraction of pesticide and veterinary drug residues from animal and agricultural crop matrices, built for the multi-residue SPME-LC-MS/MS methods.

Supporting Research

High throughput and automated solid-phase microextraction and determination by liquid chromatography-mass spectrometry for the analysis of mycotoxins in beer A fully automated, high-throughput SPME blade system processing 96 beer samples simultaneously (57 seconds/sample) with matrix-compatible HLB/PAN coatings, coupled directly to LC-MS for low-matrix-effect quantification of 9 mycotoxins with strong sensitivity and reproducibility.
Comparison of Solid-Phase Microextraction to Solvent Extraction and QuEChERS for Quantitative Analysis of Veterinary Drug Residues in Chicken and Beef Matrices Demonstrated SPME's superiority over traditional methods in extracting drugs from animal tissues. Benefits included gains in sensitivity and speed while reducing matrix effects.
Solid-phase microextraction with recessed matrix compatible coating for in situ sampling of per- and polyfluoroalkyl substances in meat A recessed, matrix-compatible SPME rod enabling in situ extraction of 18 PFAS compounds directly from intact meat tissue, paired with LC-MS/MS for simple, low-matrix-effect quantification without homogenization or solvent-heavy cleanup.

Pharmaceutical & Bioanalysis

Assaying drug compounds and metabolites directly from plasma, blood, and other biological matrices with biocompatible coatings, built for direct-immersion SPME-LC-MS/MS bioanalysis with minimal sample prep.

Supporting Research

Therapeutic drug monitoring of tranexamic acid in plasma and urine of renally impaired patients using solid phase microextraction A high-throughput SPME-LC-MS/MS method achieving 22-second-per-sample processing of tranexamic acid in plasma and urine, applied to profile 49 renally impaired cardiac surgery patients and directly informing a corrected clinical dosing schedule.
Determination of free concentration of endocannabinoids in brain tissue A negligible-depletion HLB-SPME method coupled to LC-MS/MS that measures the free (unbound) concentration of endocannabinoids in brain tissue without disturbing the analyte-protein binding equilibrium, enabling calculation of tissue protein-binding percentages and total concentrations from small sample volumes.
Nonexhaustive microextraction as a step toward more sustainable chemical analysis in the field and the clinic A perspective outlining how nonexhaustive SPME—coupled with GC-MS, LC-MS, or direct MS—offers a greener, faster alternative to exhaustive extraction methods, comparing solvent use, throughput, and matrix effects across LLE, SPE, SPME, and coated blade spray workflows for field and clinical applications.

Direct-to-MS SPME

SPME devices can also be desorbed and ionized directly into a mass spectrometer, skipping chromatographic separation entirely. Approaches like coated blade spray and direct analysis in real time deliver results in minutes for high-throughput screening, and pair naturally with LC-MS/MS for confirmatory follow-up when it's needed.

Supporting Research

Building a Bridge Between Ambient MS and LC-MS by Non-Exhaustive Microdesorption Sequential SPME strategy that combined coated blade spray-MS screening directly followed by LC-MS confirmation, using the same sample that was prepared only once. This was applied to the analysis of 53 prohibited substances in urine, with exceptional figures of merit.
Coated blade spray-mass spectrometry for rapid screening: A tutorial A comprehensive tutorial on coated blade spray-MS, covering practical implementation strategies for SPME-based direct-to-MS approaches that enable rapid, chromatography-free screening in complex matrices like blood, plasma, and urine.
High-throughput quantification of drugs of abuse in biofluids via 96-solid-phase microextraction–transmission mode and direct analysis in real time mass spectrometry High-throughput 96-sample extraction coupled to DART-MS for rapid, chromatography-free screening and quantification of opioids in urine and plasma. Demonstrated good accuracy and sensitivity in less than 60 seconds per sample (from sample preparation to final analysis).

In-vivo Analysis

SPME is used for minimally invasive component extraction from live tissue, enabling real-time analysis.

Supporting Research

Minimally Invasive Chemical Biopsy Needle with Self-Wettable Extraction Phase For In Vivo Tissue Sampling During Medical Procedures Minimally invasive SPME-LC-MS/MS analysis of anti-cancer drugs measured in human lung during perfusion.
In vivo profiling of phytocannabinoids in Cannabis spp. varieties via SPME-LC-MS analysis A minimally invasive SPME method using biocompatible C18 probes directly inserted into growing Cannabis inflorescences, coupled to LC-Q-TOF-MS to isolate and quantify five acidic phytocannabinoids and track their composition across plant varieties and growth stages without harvesting.

SPME Applications

Environmental Analysis

Supporting Research

Agricultural Residue Testing

Supporting Research

Pharmaceutical & Bioanalysis

Supporting Research

Direct-to-MS SPME

Supporting Research

In-vivo Analysis

Supporting Research